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文献和实验(determine experimentally). A dilution series of 1:100 to 1:10,000 should cover it. Incubate at RT for 1 hr or longer, depending on the antibody. Sometimes an overnight incubation is helpful. Jon''s anti-actin Guinea pig antibodies from animal#1 are best
) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
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