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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
-20℃
- 适应物种:
Human, Mouse, Rat
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB,IHC,IF/ICC
- 浓度:
~1mg/ml
- 抗体名:
Anti-Alkaline Phosphatase, Tissue Non-Specific antibody ,应用: WB,IHC,IF/ICC,反应: H, M, R,HY- ab126820 -50ul
- 规格:
50ul
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
One of the most common methods in immunohistochemistry involves the use of an antibody to the antigen of interest detected indirectly with an enzymelabeled antispecies secondary antibody. The enzyme catalyzes the formation of a colored
Blocking of unwanted non-specific staining
that may affect your staining. Blocking of endogenous Alkaline Phosphatase is done by exploiting the differential sensitivity of calf intestinal AP, used as a reporter, and leukocyte AP. This latter is inhibited, while the former is not by Levamisole 1 mM
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