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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
|
| Alternate Names |
TCR-β; β-TCR;TCR-β chain
|
| Gene ID |
21577
|
| Clone No |
H57-597 (HB218)
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Isotype Control |
PE Armenian Hamster IgG Isotype Control[PIP]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
通过 TCR 与 MHC 多肽复合物间的特异性识别作用,可以对抗原特异性 T 细胞进行检测和分离。然而,当 MHC 多肽复合物为单体状态时,其与 T 细胞的结合并不稳定。若将其四聚体化后,其与 TCR 的结合可保持在一个稳定的状态,成为检测的工具。德国 IBA Lifesciences 研发的 MHC I STREPTAMERS® 技术,利用了这一特点,将传统的链霉亲和素改进为 Strep -Tactin®,搭配荧光标记或纳米磁珠,与多聚化的低亲和力MHC I-Streps 形成复合
免疫斑点检测(ELISPOT)、单细胞 PCR 等,MHC 四聚体分析技术具有以下优势:高灵敏度:可检测血液中低丰度(≤1%)的抗原特异性 T 细胞高特异性:极大提高了 TCR 与 MHC-抗原多肽复合物结合的特异性稳定性好:检测结果一致性好,可重复性高多样化/个性化:可定制,合成特异的抗原多肽片段,组合成各种 T 细胞选择性 MHC 四聚体定性/定量分析:可结合流式细胞术实现对抗原特异性 T 细胞群的定性/定量分析荧光标记选择灵活: 可选用 PE 或者 APC 标记HelixGen MHC 四聚体试剂染色步骤广州
KitCD2-PECD3-FITC/PE/APCCD4-FITC/PE/APCCD6-FITC/PE/APCCD8-FITC/PE/APCCD25-Biotin/PECD30-PECD62L-FITC/PE/APCAnti-CRTH-2- Biotin/PEAnti-TCRα/β-PE/APC细胞因子分泌细胞IFN-r Secretion Assay Cell Enrichment and Detection Kit(PE)IL-2 Secretion Assay Cell Enrichment
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