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- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100μg
请联系华韵客服获取说明书。
产品简介:
| Background |
CD10 is a 100 kD neutral endopeptidase and a member of the metalloprotease family. It is a type II transmembrane protein also known as common acute lymphoblastic leukemia antigen (CALLA), enkephalinase, and neprilysin. CD10 is expressed on B cell precursors, T cell precursors, and neutrophils. CD10 is involved in B cell development and has been shown to bind opioid enkephalins, bradykinin, angiotensins I and II, and other biologically active peptides.
|
| Alternate Names |
CALLA;CD10;MME;NEP;Neprilysin;Neutral endopeptidase;SFE
|
| Swissprot |
P08473
|
| Gene ID |
4311
|
| Clone No |
CB-CALLA
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Purified Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Generation of Dendritic Cells from Lymphoid Precursors
-1, the transcription factors Pax5 and Ikaros, and represent the earliest recognizable human B-cell precursor identified in human BM (7 ,8 ). BM cells of CD34+ Lin- CD10+ phenotype can differentiate into antigen-presenting DC under the appropriate culture conditions
; the more the better) with 0.25-0.5 μg anti-PAK antibody per 100 μg of lysate for 1.5hrs at 4oC with gentle rocking. 6. Add 25 μl protein A-, protein G-, or protein A/G-beads and incubate for 30min at 4oC with gentle rocking. 7. During this incubation, make
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
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