- ￥4290
- 华韵生物(Huayunbio)
- HFC2048-100Tests
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.
|
| Alternate Names |
CD49 antigen-like family member D;CD49d;ITGA4;Integrin alpha-4;Integrin alpha-IV;VLA-4 subunit alpha
|
| Swissprot |
P13612
|
| Gene ID |
3676
|
| Clone No |
BU49
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
FITC Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including
Dual- and Triple-Color Fluorospot
functional antibody pairs are derived from a few species. Another strategy being employed for triple staining is the use of additional tag/anti-tag systems other than FITC/anti-FITC (Fig. 1). 实验试剂 1. Blocking reagent after coating: 10
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
.1 (A ) A grayscale flatfield image (single 256 × 256–pixel frame). (B ) 2 × 2 flatfield correction frames. (C ) A human pluripotent stem cell colony (2 × 2 montage). (D ) The same montage in (C) after flatfield correction using the flatfield correction image in (B
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