![流式抗体/ FITC Anti-Mouse IL-17A Antibody[17F3]，HFC3886-100μg产品图](https://img1.dxycdn.com/p/s14/2025/0515/448/0006758882796482291.jpg)
- ￥2340
- 华韵生物(Huayunbio)
- HFC3886-100μg
- 2025年07月16日
- FCM
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- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
The 17F3 monoclonal antibody reacts with mouse IL-17A a 15-20 kDa cytokine expressed by Th17 cells, γδ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells. IL-17A has pleiotropic effects in immunoregulation and inflammation. It plays an important role in anti-microbial and chronic inflammation by inducing cytokine and chemokine production, neutrophil influx, and the production of antibacterial peptides but it is also an inflammatory mediator in the development of autoimmune diseases including rheumatoid arthritis, asthma, multiple sclerosis, and psoriasis. The 17F3 antibody has been shown to neutralize IL-36A in vivo.
|
| Alternate Names |
Ctla8;Il17a;Interleukin-17A
|
| Swissprot |
Q62386
|
| Gene ID |
16171
|
| Clone No |
17F3
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
ICFCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
FITC Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
FITC
|
| Conjugation Information |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
回收率优于商业试剂盒。该方法亦适用于tdRFP报告小鼠——在EAE模型中，91%的中枢浸润CD4⁺ T细胞仍保持RFP阳性，并可同步检测RORγt、IL-17A和T-bet表达。 完整操作流程 本研究推荐一套标准化流程，用于在保留荧光报告蛋白的同时高效染色转录因子或细胞因子。 若需检测细胞因子 先用PMA（50 ng/mL）和离子霉素（500 ng/mL）刺激细胞4小时，并加入布雷菲德菌素A（1 μg/mL）阻断蛋白
Intracellular Immunofluorescent Staining for Flow Cytometry
mouse PEC mIFNγ (100ng/ml) (2hr)/LPS (100ng/ml) (22hr) 2hr/22hr - Brefeldin A C17.8 IL-17A NEW! CD4+CD25- T cells from mouse spleen plus day 10 bone marrow-derived
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