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- ￥1430
- 华韵生物(Huayunbio)
- HFC3825-100μg
- 2026年09月24日
- FCM
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- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
100μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
|
| Alternate Names |
Fc gamma receptor;Fc gamma receptor 3;FcγRIII
|
| Swissprot |
P08637;O75015;
|
| Gene ID |
941
|
| Clone No |
3G8
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM;Block
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Purified Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Unconjugated
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including
、ionomycin和Brefeldin A，其中前两者协同激活细胞，后者抑制蛋白转运，从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体，易非特异性结合抗体的Fc段，导致假阳性信号。为解决此问题，菲恩生物提供Purified Anti-Mouse CD16/32 Antibody（FNab30106）用于小鼠样本封闭，；Human Fc Receptor Blocking reagent（K128）用于人样本封闭。关键在于必须选择与样本
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