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- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
100Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
|
| Alternate Names |
CDw44;Epican;HUTCH-I;LHR;PGP-I
|
| Swissprot |
P16070
|
| Gene ID |
960
|
| Clone No |
Hermes-1
|
| Host |
Rat
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE/Cyanine5 Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE/Cyanine 5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验避光染色30分钟至过夜。 最后用相同破膜缓冲液洗涤两次,重悬后通过流式细胞仪采集数据。该方案兼顾报告荧光稳定性与核/胞质蛋白染色效率,适用于多参数精细免疫表型分析。 菲恩生物提供专业流式配色设计与高质检测试剂,优化多色方案,降低干扰,提升检测精准度,助力免疫细胞精细分选,赋能免疫学、肿瘤免疫及自身免疫疾病研究。欢迎合作,共促创新! 以下是菲恩生物推荐的流式抗体搭配方案 种属 细胞群 流式抗体搭配 货号 Human T/B/NK细胞群检测 CD
Elabscience|肺泡巨噬细胞研究必看:LPS诱导急性肺炎模型制备与检测全攻略
抗体[X54-5/7.1] E-AB-F09792C FITC 标记小鼠IgG1,κ同型对照抗体[MOPC-21] E-AB-F1120D PE 标记抗小鼠Ly-6G/Ly-6C(Gr-1)抗体[RB6-8C5] E-AB-F0991H PE/Cyanine7 标记抗小鼠CD11c抗体[N418] AN00629J PerCP/Cyanine5.5 标记抗小鼠Siglec-F/CD170抗体[S17007L] E-AB-F0997A 纯化抗小鼠CD16/32抗体[2.4G
Elabscience | BV2细胞M1极化造模与流式检测,这份Protocol请收好
Buffer(E-CK-A107)或PBS缓冲液重悬细胞沉淀,调整细胞密度为1-10×106个/mL; (3)取100 μL细胞悬液,每组加入2 μL Purified Anti-Mouse CD16/32 Antibody(E-AB-F0997A),轻轻吹打混匀,室温避光孵育10 min封闭细胞表面的Fc受体; (4)加入各流式抗体(5 μL/test),轻轻吹打混匀,4℃避光孵育30 min; (5)孵育完成后,每管加入1 mL Cell Staining Buffer(E-CK-A107)轻轻
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