- ￥1235
- 华韵生物(Huayunbio)
- HFC3673-50μg
- 2025年07月10日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
50μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
|
| Alternate Names |
B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;MS4A1
|
| Swissprot |
P11836
|
| Gene ID |
931
|
| Clone No |
2H7
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG2b, κ
|
| Isotype Control |
AF/LE Purified Mouse IgG2b, κ Isotype Control[MPC-11]
|
| Conjugation |
None (AF/LE)
|
| Storage Buffer |
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验细胞的生长抑制，利用裸鼠肿瘤模型测定二者的体内抑瘤活性。结果 pYZF1cd20和pYZcpp3的表达产物，经分离纯化获得具有CD20特异结合活性的Fab’和F（ab）2片段。体外活性实验证明Fab’和F（ab）2可以特异性的与CD20+B淋巴瘤细胞结合，能竞争性抑制HI47与CD20分子的结合，F（ab）2竞争性结合CD20分子的强度大于Fab’；均能抑制B淋巴细胞瘤增殖，而且F（ab）2抑制B淋巴细胞瘤增殖的能力高于Fab’，F（ab）2对Raji和Daud细胞的IC50（22.8μg/ml
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
7211011 小鼠淋巴细胞分离液 达优 100339 Ultra-LEAF™ Purified anti-mouse CD3ε Antibody BioLegend 102115 Ultra-LEAF™ Purified anti-mouse CD28 Antibody BioLegend CD3/CD28 Streptamer® 多聚体法： 货号 名称
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