- ￥1235
- 华韵生物(Huayunbio)
- HFC3626-50μg
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
50μg
请联系华韵客服获取说明书。
产品简介：
| Background |
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
|
| Alternate Names |
B-cell IgG differentiation factor;B-cell growth factor 1;BSF-1;IGG1 induction factor;IL-4;Interleukin-4
|
| Swissprot |
P05112
|
| Gene ID |
3565
|
| Clone No |
MP4-25D2
|
| Host |
Rat
|
| Reactivity |
Human
|
| Application |
ICFCM;Neut
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
AF/LE Purified Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
None (AF/LE)
|
| Storage Buffer |
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Detection of Class Switching and IgE Synthesis in Human B Cells
events leading to IgE synthesis. This chapter will illustrate how human B cells (na�ve or memory) can be purified, stored, and cultivated in medium that is permissive for IgE synthesis and stimulated with IL-4 or IL-13 and CD40 activation
Intracellular Immunofluorescent Staining for Flow Cytometry
(500ng/ml) (4hr) Brefeldin A MP4-25D2 , 8D4-8 IL-5 CD4 anti-CD3 (10µg/ml, immobilized) + anti-CD28 (2µg/ml, soluble) + IL-2 (10ng/ml) + IL-4 (20ng/ml) (2d); IL-2 (10ng/ml) + IL-4 (20ng/ml
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