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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
25μg
请联系华韵客服获取说明书。
产品简介:
| Background |
Surface IgD is an important B cell differentiation marker.
|
| Alternate Names |
IGHD;Ig delta chain C region;Immunoglobulin heavy constant delta
|
| Swissprot |
P01881
|
| Gene ID |
380797
|
| Clone No |
11-26c.2a
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE
|
| Conjugation Information |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Native Chromatin Preparation and Illumina/Solexa Library Construction
19, CD36, CD56, CD123, and glycophorin A. 9. Mix well and incubate for 10 min at 4°C-8°C or on ice. 10. Add 30 µL of T cell isolation buffer per 107 cells. 11. Add 20 µL of anti-biotin microbeads (from T
Intracellular Immunofluorescent Staining for Flow Cytometry
Right: Mouse splenocytes were stimulated for 3 days with IL-2. Cells were surface stained with APC CD49d (DX5) (Cat. No. 17-5971 ) and subsequently stained intracellularly with PE GB11. Human IL-17 (eBio64CAP17): Human
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