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- 爱必信(Absin)
- 中国
- abs1850627
- 2025年07月10日
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- 库存：
99
- 供应商：
爱必信（上海）生物科技有限公司
- 规格：
100ug
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文献和实验1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Western Blotting with Horseradish Pe
(1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil for an additional 5 minutes. To reduce viscosity, the sample may be sonicated briefly or passed several times through a 26
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