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文献和实验their signal by binding to a fibroblast growth factor receptor (FGFR) which has an intracellular protein tyrosine kinase domain. The tyrosine kinase domain is activated upon FGF binding, resulting in the activation of a transcription factor by means of a signal
DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
: TBS 250 μl/well; 3x 30 seconds.8. Amplify signal by adding 100 μl/well AttoPhos Fluorescent substrate system, for 5-10 min at RT. 36 mg ofAttoPhos substrate should be mixed with 60 ml of AttoPhos buffer 24 hours prior to use. Make sure well isclean
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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