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文献和实验Porcine brain tubulin preparation
Homogenization buffer (freshly prepared) 1L PM buffer 100 mM MgATP 1L PMG Buffer 100 mM MgGTP 1M dithiothreitol (DTT) Glutamic acid sodium salt 实验设备 Waring Blender Temperature controlled ultracentrifuge
acid (sodium salt) to the tubulin solution and stir slowly until it is dissolved. Disburse the solution into 31.5 ml ultracentrifuge tubes and pair them by weight. Immerse the centrifuge tubes to the level of the liquid within in a 37oC
Fluorescence In Situ Hybridization on Early Porcine Embryos
. This chapter describes in detail a FISH protocol for overcoming this problem. Following extensive technical development, the protocol was derived and optimized for IVF porcine embryos to enable investigation of whole chromosome and subchromosomal regions
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