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文献和实验Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes
by the viral gastroenteritis unless they are protected by antibodies. In addition to the cellular receptor, porcine aminopeptidase N, the TGEV spike protein binds to sialic acid residues. We have shown that the sialic acid binding activity mediates the binding
Clarify the MT protein by centrifugation at 100,000 x g (29,000) rpm for 45 min at 4oC in a 50.2Ti rotor. At room temperature, collect the supernatant containing the MT protein, dilute it 1:1 with PMG, add GTP to 0.2 mM, disburse it into 31
Porcine brain tubulin preparation
MTs on ice for 30 minutes with gentle mixing every 5 min to allow for MT depolymerization. 17. During this time chill the ultracentrifuge and 50.2Ti rotor to 4℃. 18. Clarify the MT protein by centrifugation at 100,000 x g (29,000) rpm
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