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文献和实验Cell death detection in Xenopus embryos by ELISA
(eppendorf) for 10 min. at 4°C Remove 160 µl supernatant / embryo, avoiding pellet contamination of the supernatant Samples can be stored, if not used the same day, in aliquots at -20°C Do ELISA according to manufacturer's instructions.
Gene Delivery to the Mouse Brain with Adeno-Associated Virus
The efficient transduction of postmitotic cells by adeno-associated virus (AAV) makes it an excellent vector to deliver marker, functional, or therapeutic genes to the mammalian brain. An attractive feature of AAV is that all the viral-coding
A Mouse Model of Staphylococcus Catheter-Associated Biofilm Infection
and alter host immune responses to facilitate bacterial persistence. Here we describe a mouse model of catheter-associated biofilm infection, which can be utilized to investigate the importance of various staphylococcal determinants on disease progression
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