- ￥702
- 华韵生物(Huayunbio)
- HFC1881-25μg
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
25μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD209B, also known as SIGN-R1, is a mouse C-type lectin receptor predominantly expressed on macrophages in the spleen marginal zone and lymph nodes medulla. CD209B is a mouse homolog of human CD209/DC-SIGN and is involved in innate immune response. CD209B mediates the recognition and uptake of pathogen products, such as lipopolysaccharides (LPS), pneumococcal polysaccharides, and dextrans. CD209B has been demonstrated to facilitate the clearance of encapsulated pneumococcus by directly binding to C1q and activating complement through an immunoglobulin independent pathway.
|
| Alternate Names |
CD209;CD209 antigen-like protein B;Cd209b;DC-SIGN-related protein 1;DC-SIGNR1;OtB7
|
| Swissprot |
Q8CJ91
|
| Gene ID |
69165
|
| Clone No |
22D1
|
| Host |
Armenian Hamster
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Armenian Hamster IgG
|
| Isotype Control |
Biotin Armenian Hamster IgG Isotype Control[PIP]
|
| Conjugation |
Biotin
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Mouse B cell isolation with magnetic beads
and incubate on ice for 15min; l Negative : 1.Resuspend cells with MACS to 400 ul and 80 ul B cell Biotin each spleen,incubate on ice for 15 min; 2. Add 300 MACS and 200 anti-biotin Beads each spleen, incubate on ice for 15 min.
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm
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