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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit(cat NO:EKS-420A)?可否说一下应用的经验?注意事项等?还有,该试剂盒的说明书有几处地方不是很懂,请大家帮忙看一下: 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1
We have established a uniform procedure for the expression and purification of the cyclin-dependent kinases CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1. We attach a His6 -tag to one of the subunits of each complex and then co-express
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