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文献和实验Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures
to determine agonist-induced mucin secretion from goblet cells in human bronchial epithelial (HBE) cell cultures. It begins with primary epithelial cell culture, offers methods for purifying MUC5AC and MUC5B mucins for standards, and describes five different
Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro
surface diseases that cause impaired vision and, in advanced stages, blindness. Advances in the field of molecular biology have made it possible to study membrane mucins and their associated O-glycans in established cell culture models of human ocular
Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion
synthesize and secrete a high-mol-wt mucous glycoprotein, mucin, which is responsible for the viscoelastic property of the surface mucus layer. Secreted mucus, which is able to trap air pollutants and microorganisms, is steadily removed from the airway
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