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文献和实验Two long PCR steps: First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA. First round of the nested PCR step of the limiting dilution step to amplify single templates
ELISA原理与实验方法The advantages of the ELISA are similar to other antibody-labeled reactions which include specificity, sensitivity, inexpensiveness, and safety. Since the enzyme label is the critical portion of ELISA, its selection is very important
Long PCR Reagents and Guidelines
George Church Lab, Harvard Medical School PCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main
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