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文献和实验Interferon- Gene Therapy for the Treatment of Arthritis
Interferons were originally described as antiviral agents by Isaacs and Lindenmann (1 ). From its subtypes, fibroblast interferon (IFN-β) (2 ) was also found to have important cytostatic (3 ,4 ) as well as immunomodulatory functions
Measurement of Mouse Cytomegalovirus-Induced Interferon- with Immortalized Luciferase Reporter Cells
(MCMV) infection of immortalized fibroblasts derived from mice expressing the firefly luciferase reporter downstream of the IFN-β promoter. Common methods to determine IFN-β production, including ELISA, quantitative real-time PCR (qPCR), and transient
Transfection of Mller Cells With Type I Interferon Transgenes: Resistance to HSV-1 Infection
Gene transfer is a widely used experimental approach to determine the value of specific genes under a variety of conditions. This chapter focuses on the expression of two human antiviral genes, interferon (IFN)-α2 and IFN-β, driven
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