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文献和实验PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples
Helicobacter pylori is a curved Gram-negative bacillus that infects the human gastric mucosa. Most methods used to diagnose H. pylori require the collection of gastric samples by biopsy during gastroduodenoscopy. A positive culture
问:现在我用的南京建成的试剂盒来测脂肪酶,可是结果可以说是乱七八糟,一点也不稳定,这个问题也咨询过几位师兄,也有类似情况发生。不知道各位战友有没有碰到过这种情况?可有解决办法或者其他的测定方法?能否传授一下小弟?不胜感激! 答:我也用过南京建成的试剂盒,结果和你一样,看来试剂盒测脂肪酶还很不成熟。你可用常规方法(聚乙烯醇橄榄油乳化法),方法简单可靠,到处都能查到此方法。 答:乙烯醇的办法我试过,用国产试剂效果还是不好。建议用sigma的橄榄油,不过价格很贵。 实验方法如下:Lipase
Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice
are common and impact of concurrent EHS infection on Helicobacter pylori -induced gastric pathology has been demonstrated. PCR of DNA isolated from gastric tissue is the most sensitive and efficient technique to confirm the H. pylori infection
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