- ￥636 - 3499
- DIMA BIOTECH/缔码生物
- 武汉
- PME101186
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期：
12个月
- 英文名：
Recombinant Human BTK Protein with C-terminal 3×Flag tag
- 库存：
In Stock
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
10μg/50μg/100μg
| 规格： | 10μg | 产品价格： | ￥636.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2333.0 |
| 规格： | 100μg | 产品价格： | ￥3499.0 |
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | BTK |
| Uniprot ID | Q06187 |
| 别名 | AGMX1;AT;ATK;BPK;IGHD3;IMD1;PSCTK1;XLA |
| 背景 | The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013] |
| 产品英文名称 | Recombinant Human BTK Protein with C-terminal 3×Flag tag |
| 分子结构 | BTK(Met1-Ser659) 3×Flag tag |
| 分子量 | The protein has a predicted molecular mass of 79.2 kDa after removal of the signal peptide. |
| 纯度 | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| 标签 | C-3×Flag Tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 货期 | In Stock |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 应用范围 | Research use only |
Figure 1. Human BTK Protein, Flag Tag on SDS-PAGE under reducing condition.
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
大阪大学蛋白质研究所藤井勇树、高木淳一东北大学大学院医学系研究科金子美华、加藤幸成 前言在分析蛋白质的功能时最重要的是如何得到高纯度的蛋白。现今提纯蛋白最常用的方法是亲和标签系统。被统称为「Epitope Tag」的肽标签以及其单克隆抗体组成的系统有FLAG、HA、Myc等多种标签。但是，每个标签系统都有优缺点，并不存在完美的标签系统。于是，在克服了众多难关后我们终于开发出有超高亲和性的新型亲和标签系统“PA Tag System”1）。 PA tag的起源Epitope Tag
【Jounal club】常用标签Tag的比较: 纯度，收率，载量，成本等
poor purifiation from yeast, Drosophila, and HeLa extracts. The CBP tag produced moderate purity protein from E. coli, yeast, and Drosophila extracts, but better purity from HeLa extracts. Epitope-based tags such as FLAG and HPC produced the highest
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