Goat anti-Mouse IgG, IgM (H+L)

Goat anti-Mouse IgG, IgM (H+L) Secondary Antibody, Alexa Fluor™ 488

货号 A-10680

These goat anti-mouse IgM/IgG (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

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