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- 2025年07月15日
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- 详细信息
- 技术资料
- 库存：
现货
- 保存条件：
Store for 1 year at -20°C or for 6 months at +4°C upon receipt. Avoid exposure to light.
- 规格：
50rxns
Product Information
|
Product name |
FlexAble 2.0 CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 |
|
Assay type |
Antibody labeling |
|
Tested applications |
IF, FC, WB |
|
Species Reactivity |
Mouse IgG1 |
|
Antibody amount per labeling reaction |
0.5 µg antibody |
|
Conjugate |
CoraLite® Plus 647 |
|
Excitation / Emission maxima wavelengths |
654 nm/ 674 nm |
产品介绍
| Component | 10 rxns | 50 rxns | 200 rxns |
| CoraLite® Plus 647 FlexLinker 2.0 for Mouse IgG1 | 10 μL | 50 μL | 200 μL |
| FlexQuencher for Mouse IgG1 | 20 μL | 100 μL | 400 μL |
| FlexBuffer | 100 μL | 500 μL | 2000 μL |
保存条件
Store for 1 year at -20°C or for 6 months at +4°C upon receipt. Avoid exposure to light.
其他
Q: What is the difference between FlexAble and FlexAble 2.0 products?
A: FlexAble 2.0 has been improved to allow for more dyes per FlexLinker than the previous version of the kit. This results in an increased signal per antibody.
Q: What are the FlexLinker, FlexQuencher and FlexBuffer?
A: The FlexLinker is a small polypeptide to which dyes are covalently conjugated that can label unconjugated primary antibodies. The FlexQuencher is an Fc-containing fragment that neutralizes the excess FlexLinker. The FlexBuffer is a PBS-based buffer.
Q: What is the largest quantity I can label?
A: With a standard kit size (50 reactions), you can label 25 µg of one antibody or up to 50 different antibodies. You can easily scale up the antibody amount per labeling approach.
Q: What is the lowest concentration of my primary antibody that I can use?
A: Our protocol uses 0.5 µg of primary antibody in 7 µL, which ends up at 0.07 mg/mL. If the concentration of your antibody is lower, you can also use a larger volume than 7 µL.
Q: Can I label primary antibodies stored in BSA, glycerol, Tris buffer and/or preservatives?
A: Yes, FlexAble Antibody Labeling Kits have been validated with carriers and amine buffers. Neither BSA nor amine buffers, in any chosen concentration, interfere with the labeling. 50% glycerol as well as preservatives like sodium azide are also compatible with the kit.
Q: How many different primary antibodies can I label with one kit?
A: You can label up to 50 different antibodies with our FlexAble 50 rxn Kit, and up to 10 antibodies with our FlexAble 10 rxn Kit.
Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?
A: FlexAble labels primary antibodies with a high affinity FlexLinker. Dissociation of FlexLinker from one antibody and association to another antibody is rare. If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies.
-
FlexAble系列是Proteintech研发的一种新型抗体标记试剂盒，采用独有的FlexLinker®抗体标记技术专利，能在任何抗体缓冲液条件下，10分钟将荧光染料、酶、以及其他分子与抗体结合，极大提高流式细胞术、免疫荧光及 免疫印迹实验效率。
-
与任何品牌一抗都兼容
-
单次可标记0.5ug抗体
-
适用于任何抗体浓度和配方
-
标记过程中抗体无损失
-
两步法10分钟快速标记
-
无需额外设备或更换缓冲液
操作流程：
-
进行标记之前，所有试剂需平衡至室温。
-
1、将0.5 μg抗体与1 μL FlexLiner混合后，
-
加入不高于8 μL的FlexBuffer。
-
注意：此处需要进行抗体质量换算：抗体质量=抗体浓度X体 积。
-
2、室温避光孵育5 min
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3、加2 μL FlexQuencher
-
4、室温避光孵育5 min
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5、抗体标记完成，可直接使用，无需过滤纯化
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