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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存,一年有效。
- 英文名:
Enhanced Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO)
- 规格:
1 mL
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文献和实验Yeast mini whole cell extract using glass beads for western analysis
----------------------- Extraction Buffer : 200 mM Tris pH 8.0 150 mM Ammonium sulfate 10% glycerol 1 mM EDTA Add fresh on day of extract preparation : 2 mM DTT PMSF Benzamidine Leupeptin Pepstatin Chymostatin Protease inhibitors 0.1 M PMSF (100x) 16 mg/ml Ethanol
Yeast mini whole cell extract using glass beads for western analysis
(100X) 31 mg/ml H2O. Store frozen at -20 degrees Leupeptin (500X) 0.15 mg/ml Ethanol. Store at -70 degrees for less than 6 months Pepstatin (200X) 0.28 mg/ml methanol Store at -20 degrees. Chymostatin (2,500X) 5mg/ml DMSO
bovine serum (F2442) • 20% DMSO (D2650) • 60% ES cell media • Store freezing media at –20 °C and thaw to 37 °C or room temperature before use. Ready-to-Go Freezing media • Serum-free cyropreservation medium (C6295)
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