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- gemig
- XF-J5978
- 2025年07月15日
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产品货号：XF-J5978
产品规格：1mL*5
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
Biotin labeling and hybridization of probe to chromosomes
times more than the probe DNA. Hybridization of probe to chromosomes. Denature the mixture by placing at 75 C for 5 min, immediately chilling on ice for 5 min, and spining down the solution. Place the slides (by using
DNA Probe Purification Protocol
microgram DNA due to the limiting nucleotide concentrations used. In this case, a carrier such as yeast tRNA should be added to Buffer DP to a final concentration of 2-5 ug/mL before use to ensure binding to HiBind resin. The mixture is stable at -70℃
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