Hot Start Taq DNA Polymerase 热

DNA聚合酶，你选对了吗？

PCR(Polymerase Chain Reaction) 即聚合酶链式反应，对于常年狂奔在实验室的实验狗来说并不陌生。那么，对于 PCR 反应的核心成份，DNA 聚合酶，你选对了吗？ 常见的 DNA 聚合酶如 Taq、Phanta、Pfu、KOD、Primerstar、Klenow、Bst、Phi29 等等。根据耐热性来分可分为耐热酶和常温酶。耐热聚合酶如 Taq、Pfu、KOD、Primerstar 等，常温聚合酶包括 Klenow、Bst、Phi29 等。根据保真度来分，高保真聚合酶

使用 Platinum® Taq DNA 聚合酶 (Platinum® Taq DNA Polymerase)，对靶序列进行定性验证

实验步骤   The procedure on the following page is suggested as a guideline and starting point when using Platinum® Taq  DNA Polymerase in any PCR amplification.  Optimal reaction conditions (incubation times and temperatures

Taq酶PCR实验方法介绍

for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended