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文献和实验How long can bisulfite-treated DNA be stored-DNA Methyla
---------------------------------------------------------------------------- Jon, I resuspend my bisulfite treated DNA in fresh autoclaved TE buffer and store at -20C. I have stored such DNA upto 6 months with no detectable loss of DNA and by then I have used up my batch and I have to make
Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA
Amplification of sodium bisulfite-treated DNA is widely used to study DNA methylation. The proportion of methylated sequences of a specific DNA region in a sample can be determined by the analysis of PCR products or directly calculated from real
by adding 55 mg of hydroquinone to 50 ml of water. Add 520 ml freshly prepared 3M Sodium bisulfite (Sigma S-8890), prepared by adding 1.88 gm of sodium bisulfite per 5 ml of H2O, and adjusting pH to 5.0 with NaOH. Assure that reagents are mixed with DNA
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