- ￥2450
- AntibodySystem
- France
- RHD50003
- 2025年07月09日
- ELISA, WB
- Mouse
- Human
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AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
ELISA, WB
- 浓度：
1 mg/ml
- 靶点：
Alpha-(1,3)-fucosyltransferase 4, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Galactoside 3-L-fucosyltransferase, Fuc-TIV, FCT3A, FUT4, ELFT, FucT-IV
- 抗体英文名：
Anti-CD15/FUT4 Antibody (R3G05)
- 抗体名：
Anti-CD15/FUT4 Antibody (R3G05)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG2b |
| Conjugation | |
| Tested applications | ELISA: 1:10000, WB: 1:500-1:2000 |
| Applications | ELISA, WB |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | Alpha-(1,3)-fucosyltransferase 4, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Galactoside 3-L-fucosyltransferase, Fuc-TIV, FCT3A, FUT4, ELFT, FucT-IV |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P22083 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R3G05 |
| Note | For research use only. |
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文献和实验for this purpose. Grow cells in 200 mls of YPD at 30oC to an OD 600 of 1. Add formaldehyde to the 200 ml culture to a final concentration of 3.7. Fix for 1 hour at room temperature with shaking. Centrifuge cells at 3000 X g. Resuspend
Conjugation of Antibody to HRP
Antibody Concentrate the antibody to 5-7 mg/ml Dissolve 10 mg of SATA in 1 ml DMF (SATA:DMF) Add 3.0 ml SATA:DMF per mg of antibody Gently stir solution for 2 hours at room temperature. Dissolve 0.5 g hydroxylamine hydrochloride in 10 ml PBS
Centrifuge cells at 3000 X g. Resuspend in 200 mls Solution A. Repeat wash. Pellet cells as above and resuspend in 4 mls Solution A. Reserve 2 mls of the cell suspension. Spheroplast the remaining 2 ml of the cell suspension by addition
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