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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
1 year
- 级别:
科研级
- 库存:
99999
- 供应商:
AntibodySystem
- 宿主:
Mouse
- 应用范围:
ELISA, FCM, IF
- 浓度:
1 mg/ml
- 靶点:
Melanotropin alpha, Beta-MSH, Gamma-MSH, Beta-LPH, Melanotropin beta, Adrenocorticotropic hormone, Pro-opiomelanocortin, ACTH, CLIP, Gamma-LPH, Corticotropin-lipotropin, Alpha-MSH, POMC
- 抗体英文名:
Anti-POMC/ACTH Antibody (R2X25)
- 抗体名:
Anti-POMC/ACTH Antibody (R2X25)
- 规格:
100 μg

| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | ELISA: 1:10000, FCM: 1:200-1:400, IF: 1:200-1:1000 |
| Applications | ELISA, FCM, IF |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | Melanotropin alpha, Beta-MSH, Gamma-MSH, Beta-LPH, Melanotropin beta, Adrenocorticotropic hormone, Pro-opiomelanocortin, ACTH, CLIP, Gamma-LPH, Corticotropin-lipotropin, Alpha-MSH, POMC |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P01189 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R2X25 |
| Note | For research use only. |
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文献和实验monoclonal antibody production fusion
through the nylon filter; wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. mix 10^8 spleen cells (in 25 ml of IMDM [serum free]) with 2x10^7 myeloma cells (in 25 ml of IMDM [serum free]) in Falcon-50 ml conical tube.
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
hydrochloride (EDC) 25 µg/ml anti‐mouse IgG antibody in 10 mM sodium acetate, pH 5.0 (Biacore) 1 M ethanolamine
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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