- ￥2450
- AntibodySystem
- France
- RHB97602
- 2025年07月15日
- ELISA, FCM, IHC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
0.01M PBS, pH 7.4, 0.05% Sodium Azide.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
99999
- 供应商：
AntibodySystem
- 宿主：
Mouse
- 应用范围：
ELISA, FCM, IHC
- 浓度：
1 mg/ml
- 靶点：
Collagen alpha-1(III) chain, COL3A1
- 抗体英文名：
Anti-PIIINP/COL3A1 Antibody (R2X82)
- 抗体名：
Anti-PIIINP/COL3A1 Antibody (R2X82)
- 规格：
100 μg
| Clonality | Monoclonal |
| Species reactivity | Human |
| Host species | Mouse |
| Isotype | IgG1 |
| Conjugation | |
| Tested applications | ELISA: 1:10000, FCM: 1:200-1:400, IHC: 1:200-1:1000 |
| Applications | ELISA, FCM, IHC |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.05% Sodium Azide. |
| Concentration | 1 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Target | Collagen alpha-1(III) chain, COL3A1 |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P02461 |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Clone ID | R2X82 |
| Note | For research use only. |
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
Immunohistochemistry (IHC) of Thymus Frozen Sections
µL of 2º antibody at r.t. as before x5 min. Rinse with PBS as before. Add DAB solution at r.t. x5 min. Rinse in PBS 2x. Step slides through graded EtOH to dehydrate: 70%, 95%, 100% (x3). Toluene (or xylene) (x2). Add
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