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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
Porcine follicle-stimulating hormoneS, 10 mg/L, PHO1[174]Porcine inhibitor of carbonic anhydrase (transferrin family)S, 5 mg/L, α-MF[175]Porcine single-chain interleukin-12S, 4 mg/L,α-MF, active370Porcine leukocyte 12-lipoxygenaseI[176]Porcine lactoferrinS
folding at the expense of inter-molecular aggregation.On the other hand,MBP-tagged proteins must acquire and retain their native conformation in order to be purified on amylose affinity columns.Presumably the methods below can be adapted with few changes
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