- ￥4000
- 欣润生物
- 江苏
- IP4012
- 2025年12月07日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortalized pig liver cells
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
不是
- 细胞类型：
永生化细胞
- ATCC Number：
111
- 品系：
巴马
- 组织来源：
肝
- 相关疾病：
无
- 物种来源：
巴马猪猪
- 免疫类型：
正常
- 细胞形态：
上皮型
- 是否是肿瘤细胞：
否
- 器官来源：
肝脏
- 运输方式：
常温
- 年限：
成年
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化猪肝实质细胞简介：
产品描述：在猪正常生理和病理条件下，肝脏在能量代谢、外源物质的生物转化、基质蛋白的合成、解毒等方面发挥着极其重要的作用。肝实质细胞是指具有肝功能的基本组成单位，大约占肝脏体积及数量的80%左右，肝实质细胞具有多方面的功能，例如：蛋白质的合成和储存、分泌胆汁和解毒物质等。肝实质细胞体外常常被用作研究肝脏功能、能量代谢和肝脏疾病的良好模型。
产品货号：IP4012
产品类型: 永生化细胞类型
传代能力: 30代左右
产品形态: 上皮型
培养基：永生化猪肝实质细胞专用完全培养基，产品号码：IP4012-5
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：顺丰快递，常温T25方瓶运输
货期：1周左右货期
Albumin抗体免疫荧光染色鉴定
CK18抗体免疫荧光染色
Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination
Hepatocyte transplantation and bioartificial liver treatment are attractive alternatives to liver transplantation. The availability of well-characterized human hepatocyte lines facilitates such cell therapies. Human hepatocytes were immortalized with a retroviral vector SSR#197 expressing catalytic subunit of human telomerase reverse transcriptase (hTERT) and enhanced green fluorescent protein (EGFP) cDNAs flanked by a pair of loxP recombination targets. Then, Tamoxifen-dependent Cre recombinase was expressed in SSR#197-immortalized hepatocytes. Cre/LoxP recombination was performed in the established cells by simple exposure to 500 nM Tamoxifen for a week. Then, the reverted population of the cells was recovered by EGFP-negative cell sorting and characterized in vitro and in vivo using a pig model of acute liver failure (ALF) induced by d-galactosamine (0.5 g/kg) injection. A human hepatocyte cell line 16T-3 was established. Reverted 16-T3 cells showed the increased expression of hepatic markers in association with enhanced levels of transcriptional factors. Compared to
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文献和实验Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
技术资料