- ¥374.90 - 16999.90
- 阿拉丁
- 上海
- Ab107657
- 2026年09月16日
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- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide derived from human Histone H2A.X (AA 12-48)
- 亚型：
Rabbit IgG
- 保存条件：
-20°C储存,避免反复冻融；超低温运输
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 浓度：
见COA
- 规格：
Ab107657-100μl/Ab107657-1ml/Ab107657-50μl/Ab107657-10μl
| 规格： | Ab107657-100μl | 产品价格： | ¥1574.90 |
|---|---|---|---|
| 规格： | Ab107657-1ml | 产品价格： | ¥16999.90 |
| 规格： | Ab107657-50μl | 产品价格： | ¥974.90 |
| 规格： | Ab107657-10μl | 产品价格： | ¥374.90 |
基本信息
产品名称：Recombinant Histone H2A.X Antibody
规格或纯度：重组, ExactAb™, 已验证, 见COA
应用：WB, IF/ICC, IHC
特异性：Histone H2A.X
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：A synthetic peptide derived from human Histone H2A.X (AA 12-48)
阳性对照：WB: Mouse kidney, Rat brain, Raji, Ramos, HeLa and HEK293 lysates. IHC: Rat stomach, pancreas, lung, Human Hodgkin's lymphoma, colon cancer, Mouse spleen tissues. IF/ICC: HeLa cells.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
Format：Whole IgG
亚型：Rabbit IgG
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂：0.02% Sodium azide
浓度：见COA
储存条件：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联：Unconjugated
图片
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Recombinant Histone H2A.X Antibody (Ab107657) - Western Blot
All lanes: Recombinant Histone H2A.X Antibody (Ab107657) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Monoubiquitination of Lys-120 (H2AXK119ub) by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression.The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.
Exposure time: 11.3 s
>
Recombinant Histone H2A.X Antibody (Ab107657) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Histone H2A.X Antibody (Ab107657).
>
Recombinant Histone H2A.X Antibody (Ab107657) - IHC
Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant Histone H2A.X Antibody (Ab107657) at 1/200 dilution.
>
Recombinant Histone H2A.X Antibody (Ab107657) - IHC
Immunohistochemical analysis of paraffin-embedded Rat pancreas, using Recombinant Histone H2A.X Antibody (Ab107657) at 1/200 dilution.
>
Recombinant Histone H2A.X Antibody (Ab107657) - IHC
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using Recombinant Histone H2A.X Antibody (Ab107657) at 1/200 dilution.
>
Recombinant Histone H2A.X Antibody (Ab107657) - IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer, using Recombinant Histone H2A.X Antibody (Ab107657) at 1/200 dilution.
>
Recombinant Histone H2A.X Antibody (Ab107657) - IHC
Immunohistochemical analysis of paraffin-embedded Mouse spleen, using Recombinant Histone H2A.X Antibody (Ab107657) at 1/200 dilution.
>
Recombinant Histone H2A.X Antibody (Ab107657) - IHC
Immunohistochemical analysis of paraffin-embedded rat lung, using Recombinant Histone H2A.X Antibody (Ab107657).
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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
RNAs;Following the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution
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antibody unconjugated； 货号21337，产品描述：GAPDH Antibody；规格 50 ul / 100 ul； 货号3052，产品描述：Rabbit anti-Human IgG secondary antibody HRP conjugated； 货号300904050，产品描述：One-Hour Western kit，10 ml / bottle x 3；
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