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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Anti-V5 Affinity Gel
- 库存:
询盘
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
A743825-500μl/A743825-2ml/A743825-10ml
| 规格: | A743825-500μl | 产品价格: | ¥932.9 |
|---|---|---|---|
| 规格: | A743825-2ml | 产品价格: | ¥2795.9 |
| 规格: | A743825-10ml | 产品价格: | ¥9323.9 |
英文名:Anti-V5 Affinity Gel
纯度或规格:0
SPU:A743825
CAS:-
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
) glycerol, 5% (v/v)b-mercaptoethanol, and 0.003% (w/v) bromophenol blue. 2.2. Sodium Dodecyl Sulfate (SDS) Polyacrylamide Gel Electrophoresis (PAGE) 1. Separating buffer (4×): 1.5 M Tris-HCl, pH 8.7, and 0.4%(w/v) SDS. Store at room temperature (RT
, and 2) fractionation over BlueSepharose, which binds Wnts with high selectivity. The purificationconsists of four chromatography steps: Blue Sepharose, immobilized metal affinity chromatography (IMAC), gel filtration,and heparin cation exchange
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