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文献和实验Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Human complement component C2 is a critical factor of the classical complement pathway. Here we provide a method for the production of recombinant human C2 (rhC2) protein for research purposes. The human complement component C2 (hC
Evaluation of Complement Function by ELISA
Evaluation of total complement function in human serum is an essential component of laboratory diagnostics of the human complement system. During recent years, established hemolytic assays for classical pathway and alternative pathway
volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
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