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- 2025年07月14日
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北京诺博莱德科技有限公司
RNAstore Blood RNA Kit(For RNAlater Preserved Blood )
目录号：91510
产品简介
RNAstore Blood Total RNA Kit（离心柱）用于配套RNAlater（无液氮RNA样品保存液 Cat NO ：R115）使用。RNAstore Blood Total RNA Kit包含血液采集、稳定和运输的无液氮RNA保存液（试剂盒自带，也可以用其他公司的RNAlater）,以及RNA吸附柱技术的分离纯化组分。纯化可以使用离心机手工操作，也可以在QIAcube全自动核酸纯化仪上自动运行。该产品表现卓越，比同类产品提取的总RNA产量高一倍以上。
产品特点
适用于配套RNAlater使用，提取保存在RNAlater中的全血的总RNA。
Kit Contents and Storage
|
Kit Contents |
Storage |
50 Preps (91510-50) |
|
Lysis Solution |
4 °C |
40 ml |
|
Sodium Acetate Buffer |
RT |
5 ml |
|
Buffer RW1 |
RT |
40 ml |
|
Wash Buffer RW |
RT |
10 ml Add indicated ethanol before first use |
|
RNase-free H2O |
RT |
10 ml |
|
DNase Buffer |
–20 °C |
1.25ml x 2 |
|
RNase free DNase I |
–20 °C |
250 μl |
|
RNAfixer(RNAlater) solution |
RT |
70 ml |
|
RNA Binding Columns |
RT |
50 |
All reagents, when store in indicated temperature, are stable for 9 months.
Description
RNAstore Blood RNA Kit is designed for isolation of total RNA from blood samples stored in RNAfixer( Same as RNAlater) Solution. Blood samples can be processed immediately, or they can be stored in RNAfixer Solution(provided with the kit) for a few days at ambient temperature or for prolonged periods at -20°C prior to RNA extraction.
The Kit RNA isolation procedure consists of two parts:
1. Cell lysis in a guanidinium-based solution and initial purification of the RNA by phenol/chloroform extraction
2. Final RNA purification by solid-phase extraction on a glass fiber filter. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the Kit is ready for applications such as qRT-PCR.
Materials and Equipment to be Supplied by User:
1. Microcentrifuge capable of 13,000 x g
2. 100% ethanol, water-saturated-Phenol: Chloroform( 5:1)
3. RNase-free filter pipette tips
4. Blood collection tubes (recommended anticoagulant: potassium EDTA or sodium EDTA)
5. 1.5 or 2.0 ml microcentrifuge tubes
6. Shaking incubators or heat blocks capable of 75°C-90°C
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 30005000 x g using a swing-out rotor.Note: Ensure that the blood sample
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