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- 2025年07月15日
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文献和实验The study of developmental mechanisms in postimplantation mammalian embryos can be performed for a short term using whole-embryo culture systems (see Chapters 7 and 8 ), or for an extended period using in utero (1 ,2 ) or exo utero
Exo Ovo Culture of Avian Embryos
Exo ovo culture of avian embryos is a technique for long-term culturing of embryos outside of their own shell and shell membranes. The problem of how to gain access to the avian embryo while allowing it to grow normally has been the subject
1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract and NaOAc/EtOH ppt. 2. Prepare one S1 nuclease tube (on ice) for each time point: 15ml S1 Buffer + 0.25U S1/ml (5U). 3. Use 5ml (=0.5mg) digested plasmid (in EB) per time
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