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99
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爱必信(上海)生物科技有限公司
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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
through. Fig. 3.2. Purification of recombinant sFRP-2. A Concentrated conditioned medium fromsFRP-2/MDCK cells was applied to Hi-Trap heparin resin (bed volume, 1 mL). Sampleswere eluted with increasing NaCl concentration ( dashed line ) and protein content
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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