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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
500mg
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文献和实验Use of Diphenylamine in the Detection of Apoptosis
The diphenylamine assay is a very useful tool in measuring apoptosis by determining the percentage of fragmentation of known amounts of DNA into oligosomal-sized fragments. Another advantage of the diphenylamine assay is that apoptotic DNA
Guanidine Hydrochloride Purification of Proteins From SDS
Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M
Diphenylamine Assay of DNA Fragmentation for Chemosensitivity Testing
, and internucleosomal DNA fragmentation. A variety of methods exist to determine apoptosis along this pathway. Diphenylamine assay enables the quantitation of degraded DNA. The protocol is simple and allows manipulation of all types of cell cultures. Resulting
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