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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
10mg
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文献和实验ARS 序列(autonomous replicating sequence)
即自主复制序列, 又叫复制起点序列(replication origin sequence)。是顺式作用元件的一种。这种序列是染色体正常起始复制所必需的。所有的ARS的DNA均有一段保守序列:200bpA(T)TTTAT(C)A(G)TTTA(T)-200bp,真核生物染色体上有多个ARS序列。上下游各200个bp左右的序列是维持ARS功能所必需的。
Detection of Replication Origins Using Comparative Genomics and Recombinational ARS Assay
Effective experimental techniques are available to identify replication origin regions in eukaryotic cells. Genome-wide identification of the precise sequence elements that direct origin activity is however still not straightforward
Replication timing by comparative hybridization
in Eppendorf tubes and freeze the pellets at -20o C. 7. Extract the DNA ( smash-&-grab with glass beads); digest 1/4 to 1/3 of it with the enzyme of choice in 40-60 microliters (EcoRI works well, as it cuts the ARS-less circle just once). Split
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