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- 爱必信(Absin)
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- 2025年07月08日
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dense products have been devised. A chromogenic substrate, 5-bromo-4-chloro-3-indolyl phosphate (X-Phos), which is very similar to X-gal, can be employed for detection and leads to production of a blue precipitate (Figure 1). In addition, the tetrazolium
96-well RNA In Situ Hybridization Protocol
). To stop development of individual wells, remove staining solution and add 200ul PBT. 26. To stop entire plate, rinse plate 3X with PBT. 27. Add 200ul 70% gycerol to each well. 28. Embryos are ready to mount or dissect once they have settled
P-Glucuronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants
expressing the reporter gene within the cellular context of the explant or tissue. A qualitative hrstochemical assay to measure GUS activity is based on the substrate 5-bromo-4-chloro-3-mdolyLl3-o-glucuronide (X-Glut) ( 1 ). The GUS enzyme hydrolyzes X-Glut
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