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- 爱必信(Absin)
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- abs6111321
- 2025年07月16日
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文献和实验Gene Knockout Techniques(1) The Knockout Process
with trimethylpsoralen and UV irradiation, set up 1152 subpopulations, screen DNA made from this library for deletions in specific genes by nested PCR, and then to recover single worms carrying the deletions through a sib-selection process. We have made various
for genome engineering mediatedby CRISPR-Cas9 system. 3.3 Genome Cleavage Analysis 1. Genomic DNA extraction. Extract genomic DNA from transfected cells using theQuickExtract DNA extraction kit following the manufacturer’srecommended
The Polymerase Chain Reaction (PCR)
a means of selectively amplifying a particular segment of DNA. • The segment may represent a small part of a large and complex mixture of DNAs:e.g. a specific exon of a human gene. • It can be thought of as a molecular photocopier. A Molecular
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