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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
100T
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文献和实验1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20℃) cyclin staining
secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
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