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文献和实验Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
RUVKUN Antibody Staining Protocol revised 10/29/90
hours at RT. 13. Wash for 4-24 hours at RT in Buffer B (usually do 5 quick washes and then 2-3 hours later do 1-2 more). 14. Incubate with 2 in Buffer A, 4-24 hours, RT (1:200 FITC-coupled goat anti-rabbit antibody for lin-14
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
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