AF647 Rat anti-Mouse I-A/I-E

Transgenic Mouse and Transgenic Rat Outline

-galactosidase or human placental alkaline phosphatase. You may use a non-mouse protein such as a human protein or an epitope tagged (i.e. HA, FLAG or myc) protein that can be identified with an antibody that doesn't bind to mouse proteins. For further discussion

Immunofluorescent Staining of Mouse and Rat Leukocytes

secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas