PE-Cy7 Armenian Hamster anti-M

In Vitro T Cell Activation

proliferation. What You Need Materials 1X sterile PBS Anti-mouse CD3e, 145-2C11: Functional Grade, Cat. No. 16-0031 , or Purified, Cat. No. 14-0031 Supplemented RPMI-1640 click

T-Cell Activation Using mAb to CD3

splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16

T cell Activation Protocol

cell suspension   20 minutes to set up the assay   2-4 days incubation         Method:       ANTIBODY COATING OF THE ASSAY PLATE:   1) Prepare a 5-10 ug/ml solution of anti-CD3e (145-2C11