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- 爱必信(Absin)
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- 2025年07月10日
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文献和实验Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
Th17 Polarization of Mouse CD4 Cells
in complete medium and use your favorite method to isolate CD4 cells. Check out Biocompare.com to find useful kits. 2. Th17 Polarization of CD4 cells 1) Coat 60×15 mm of plastic petri dishes with anti-mouse CD3ε, clone 145-2C11(2&mu
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