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- 2025年07月12日
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爱必信（上海）生物科技有限公司
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文献和实验Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
Western Blotting with Monoclonal Antibodies
for 30 minutes with agitation, changing the wash buffer every 3? minutes. Dilute the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies).
Flow Cytometric Analysis Of Bcl Family members
minutes to prevent non-specific antibody binding. Cells were washed thrice in FACS buffer, incubated with the relevant antibody or relevant isotype matched control antibodies at 4„aC for 40 minutes. This was followed by three washes in FACS buffer
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